Coding

Part:BBa_K2768003:Design

Designed by: Wenfei Yu   Group: iGEM18_USTC   (2018-09-18)


6-hlno: 6-hydroxy-L-nicotine oxidase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 459
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We did the codon optimization refer to E.coli K12


Source

6hlno: https://www.ncbi.nlm.nih.gov/protein/YP_007988777.1 plasmid pAO1: https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Tree&id=208964&lvl=3&keep=1&srchmode=1&unlock

References